biotin labeled meaning in English
生物素标记的
Examples
- Anti - igg antibody was immobilized onto the surface of gold electrode modified with a thin layer of protein a . biotin labeled anti - igg antibody and hrp - streptavidin were premixed to form network complex to be used as probes for amplifying the sensing response of antibody - antigen interaction
抗体通过单层蛋白a固定到金电极表面,预先处理好的生物素化抗体和辣根过氧化物酶( hrp )标记的亲和素形成的网状复合物作为免疫反应信号的放大探针。 - Surface plasmon resonance immunosensor is a relatively new immunoassay technique and has been receiving more and more attention in recent years . however , a major disadvantage of spr for bioanalytical applications is that low concentration or low molecular mass analytes could not be detected directly . therefore , it is a challenging task to develop strategies for improving the detection limit sensitivity of spr . in this paper , authors present a novel strategy for improving the sensitivity of spr immunosensing using streptavidinbiotinylated antibody complex . it is proven that the amplification strategy causes a dramatic improvement of the detection sensitivity . this amplification strategy is based on the construction of a molecular complex between streptavidin and biotin labeled protein . the complex can be formed in a crosslinking network of molecules so that the amplification of response signal will be realized due to the big molecular size of complex
将链霉亲和素-生物素系统用于表面等离子体共振免疫传感的信号放大，实时检测了人免疫球蛋白g higg的蛋白浓度。发生免疫反应的传感片和生物素化抗体反应后，传感片表面的一层生物素分子随后与链霉亲和素-生物素化抗体复合物中的链霉亲和素的活性位点发生亲和反应，从而使传感片表面特异健合的物质质量显著增加，大大提高了免疫检测的灵敏度和检测限。免疫反应经放大后，可检测0 . 00510g ml浓度区间内的higg 。